Investigating antibacterial activity of Satureja bachtiarica against coagulasenegative staphylococcus strains isolated from hospitalized patients in ICU,Tehran-Iran

Document Type : Original Article

Authors

• Niloofar Shadalooei ¹
• Mehdi Goudarzi ²

¹ Department of Pharmacognosy, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran - Iran (IAUPS)

² Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Given the inappropriate use of antibiotics and prevalence of resistant bacteria, there is urgent
need for antibacterial drugs that have fewer side effects than antibiotics. Satureja bachtiarica
is a medicinal plant which had many uses in traditional medicine. In this research, the ethanol
extract of leave of Satureja bachtiarica is tested on coagulase-negative staphylococcus strains.
Materials and Methods
In this experimental study, after collecting and drying the plant extract, the ethanolic extract of
the plant was extracted by soxhelet method. Then, the antibiotic resistance pattern of coagulasenegative
staphylococcus strains to Cefoxitin, Tobramycin, Kanamycin, Amikacin, Gentamicin,
was assessed. To evaluate the antibacterial activity, the minimum inhibitory concentration was
assessed by using micro dilution broth method.
The results of this study showed that the antimicrobial effect of Satureja extract is concentration
dependent. The results of the antimicrobial activity of extracts, indicate that alcoholic extract
in all dilutions have an antimicrobial effect on the coagulase-negative staphylococcus strains.
The results show that coagulase-negative staphylococcus strains have highest resistance against
Cefoxitin (0.39 mg/ml) and highest sensitivity to Amikacin (0.035 μl/ml). However, in 200 and
100 mg/ml concentrations the extract has higher antibacterial effect.
According to the results of this study, ethanol extracts of leave of Satureja bachtiarica had
growth inhibitory effect on coagulase-negative staphylococcus strains. Therefore this plant has
the potential to be evaluated as an alternative or adjunct to antibiotics to treat coagulase-negative
staphylococcus infections.

Keywords

• Satureja bachtiarica
• Coagulase-negative staphylococci
• antimicrobial resistance

Main Subjects

• Pharmaceutical Microbiology

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