The toxicity of zinc oxide on blood factors of Huso huso and Cyprinus carpio

Document Type : Original Article

Authors

• Shahedeh Mohammadnezhad ¹
• Fatemeh Shariati ¹
• Abdolkarim Keshavarz ¹
• Habib Vahabzadeh ²

¹ Department of environment, Lahijan Branch, Islamic Azad University, Lahijan, Iran

² Department of fisheries and aquaculture, Lahijan Branch, Islamic Azad University, Lahijan, Iran

Abstract

In this research, acute toxicity (LC) of zinc oxide nanoparticle on Beluga (Huso huso) and
common carp (Cyprinus carpio) was studied. The experiment was conducted using the
Organization for Economic Co-operation and Development (No. 202) method. For both
species, 96hLC10 was determined to be more than 200 mg/l. To assess the effects of zinc oxide
nanoparticles on blood factors, leukocyte index count (Lymphocyte, Monocytes, Neutrophils,
Eosinophil, and Basophils) was performed, and the results were analyzed using IBM SPSS
software version. According to the results and based on the ANOVA test, there is no significant
difference among mean values of leukocyte index responses (p>0.05), and one should note
that the amount of lymphocyte had decreased while that of neutrophils and monocytes had
increased. Furthermore, with regard to concentration levels, this decrease or increase has not
followed a systematic trend. In Beluga based on ANOVA test, there is a significant difference
between mean values of lymphocytes and neutrophils (p<0.05). Again, compared to control,
lymphocyte count increased while that of neutrophils and eosinophil decreased, and this too, did
not follow a systematic trend. Finally, concentration to 200 mg/l ZnO did not cause mortality in
Huso huso and Cyprinus carpio. Eventually, decline of body resistance against nano zinc oxide
was observed in these species through blood cells study.

Keywords

• Nano Zinc Oxide
• Huso huso
• Cyprinus carpio
• Blood factors
• Acute toxicity

Main Subjects

• Toxicology

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